Mahmoud Ahmed

Postdoc - Biomedicine

Hierarchical Regulation of Autophagy During Adipocyte Differentiation


Journal article


Mahmoud Ahmed, Trang Huyen Lai, Trang Minh Pham, Sahib Zada, Omar Elashkar, Jin Seok Hwang, Deok Ryong Kim
PLoS ONE, vol. 17(1), 2022 6, pp. e0250865


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APA
Ahmed, M., Lai, T. H., Pham, T. M., Zada, S., Elashkar, O., Hwang, J. S., & Kim, D. R. (2022). Hierarchical Regulation of Autophagy During Adipocyte Differentiation. PLoS ONE, 17(1), e0250865.

Chicago/Turabian
Ahmed, Mahmoud, Trang Huyen Lai, Trang Minh Pham, Sahib Zada, Omar Elashkar, Jin Seok Hwang, and Deok Ryong Kim. “Hierarchical Regulation of Autophagy During Adipocyte Differentiation.” PLoS ONE 17, no. 1 (December 6, 2022): e0250865.

MLA
Ahmed, Mahmoud, et al. “Hierarchical Regulation of Autophagy During Adipocyte Differentiation.” PLoS ONE, vol. 17, no. 1, Dec. 2022, p. e0250865.


Abstract

We previously showed that some adipogenic transcription factors such as CEBPB and PPARG directly and indirectly regulate autophagy gene expression in adipogenesis. The order and effect of these events are undetermined. In this study, we modeled the gene expression, DNA-binding of transcriptional regulators, and histone modifications during adipocyte differentiation and evaluated the effect of the regulators on gene expression in terms of direction and magnitude. Then, we identified the overlap of the transcription factors and co-factors binding sites and targets. Finally, we built a chromatin state model based on the histone marks and studied their relation to the factors’ binding. Adipogenic factors differentially regulated autophagy genes as part of the differentiation program. Co-regulators associated with specific transcription factors and preceded them to the regulatory regions. Transcription factors differed in the binding time and location, and their effect on expression was either localized or long-lasting. Adipogenic factors disproportionately targeted genes coding for autophagy-specific transcription factors. In sum, a hierarchical arrangement between adipogenic transcription factors and co-factors drives the regulation of autophagy during adipocyte differentiation.